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Analysis of Phylogenetic Relationships of Main Citrus Germplasms Based on Ftir Spectra of Petals



To develop a quick, accurate and reliable technique for studying phylogenetic relationship of Citrus, FTIR (Fourier transform infrared spectroscopy) technique was used. The petals spectra of eighteen varieties of citrus germplasms were investigated by FTIR. Pretreatment methods of raw spectra (2000201350000A0cm00A0221200A01) were composed of baseline correction, normalize and first derivative (Savitzky-Golay). We used One-way ANOVA (analysis of variance) and Tukey2019s HSD (honestly significant difference) to extract effective wave bands, where the spectral absorbance values of different citrus germplasms were significantly different. The results showed that 2000223C183100A0cm00A0221200A01, 1763223C159500A0cm00A0221200A01, 1517223C109000A0cm00A0221200A01, 1035223C102400A0cm00A0221200A01, 950223C93500A0cm00A0221200A01, 861223C78400A0cm00A0221200A01, 744223C72100A0cm00A0221200A01 and 653223C60800A0cm00A0221200A01 were the effective wave bands. HCA (hierarchical cluster analysis) was adopted to classify citrus germplasms based on the above eight effective wave bands. It was found that eighteen citrus varieties were classified into six subgroups. The results of classification and citrus phylogenetic relationships between six subgroups were consistent of results from Morphology, Biochemistry, Cytology and Molecular Biology. The overall results demonstrated that fourier transform infrared spectroscopy technique with One-way ANOVA and Tukey2019s HSD and hierarchical cluster analysis model were promising for the rapid, accurate and reliable classification for citrus as well as studying citrus phylogenetic relationship.



Total Pages: 10
Pages: 295-304


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Volume: 21
Issue: 3
Year: 2015

Cite this document


Barkley, Noelle A. et al. "Assessing Genetic Diversity and Population Structure in a Citrus Germplasm Collection Utilizing Simple Sequence Repeat Markers (SSRs)." Theoretical and Applied Genetics 112.8 (2006): 1519-1531. Crossref. Web.

Becker, K. et al. "Fourier-Transform Infrared Spectroscopic Analysis Is a Powerful Tool for Studying the Dynamic Changes in Staphylococcus Aureus Small-Colony Variants." Journal of Clinical Microbiology 44.9 (2006): 3274-3278. Crossref. Web.

BordenV. M. H. (2005). Identifying and analyzing group differences. In M. A.Coughlin (Ed.), Intermediate/advanced statistics in institutional research (pp. 132–168). Tallahassee, FL: Association for Institutional Research.

Carvalho, Sabrina et al. "Hyperspectral Reflectance of Leaves and Flowers of an Outbreak Species Discriminates Season and Successional Stage of Vegetation." International Journal of Applied Earth Observation and Geoinformation 24 (2013): 32-41. Crossref. Web.

Dass H. Indian Journal of Experimental Biology

DreyerD. L., WatermanP. G. & GrundonM. F. (1983). Chemistry and chemical taxonomy of the Rutales (p. 215). London: Academic Press.

Federici, C. T. et al. "Phylogenetic Relationships Within the Genus Citrus ( Rutaceae ) and Related Genera as Revealed by RFLP and RAPD Analysis." TAG Theoretical and Applied Genetics 96.6-7 (1998): 812-822. Crossref. Web.

GERASIMOWICZ, W. V., and D. M. BYLER. "CARBON-13 CPMAS NMR AND FTIR SPECTROSCOPIC-STUDIES OF HUMIC ACIDS." Soil Science 139.3 (1985): 270-278. Crossref. Web.

Gmitter F. Plant Breeding Reviews

Guo S. Spectroscopy and Spectral Analysis

Guo S. Spectroscopy and Spectral Analysis

Liao, Qiuhong et al. "Cluster Analysis of Citrus Genotypes Using Near-Infrared Spectroscopy." Intelligent Automation & Soft Computing 19.3 (2013): 347-359. Crossref. Web.

Malik, M. N., R. W. Scora, and R. K. Soost. "Studies on the Origin of the Lemon." Hilgardia 42.9 (1974): 361-382. Crossref. Web.;2-1

Movasaghi, Zanyar, Shazza Rehman, and Dr. Ihtesham ur Rehman. "Fourier Transform Infrared (FTIR) Spectroscopy of Biological Tissues." Applied Spectroscopy Reviews 43.2 (2008): 134-179. Crossref. Web.

Naumann, Annette, Gregor Heine, and Rolf Rauber. "Efficient Discrimination of Oat and Pea Roots by Cluster Analysis of Fourier Transform Infrared (FTIR) Spectra." Field Crops Research 119.1 (2010): 78-84. Crossref. Web.

Paradkar, M?M, S Sivakesava, and J Irudayaraj. "Discrimination and Classification of Adulterants in Maple Syrup with the Use of Infrared Spectroscopic Techniques." Journal of the Science of Food and Agriculture 83.7 (2003): 714-721. Crossref. Web.

Torres, Andrew M., Robert K. Soost, and Ulrike Diedenhofen. "LEAF ISOZYMES AS GENETIC MARKERS IN CITRUS." American Journal of Botany 65.8 (1978): 869-881. Crossref. Web.

Wang, H.P., H.-C. Wang, and Y.-J. Huang. "Microscopic FTIR Studies of Lung Cancer Cells in Pleural Fluid." Science of The Total Environment 204.3 (1997): 283-287. Crossref. Web.

WengS. (2010). Fourier transform infrared spectroscopy (p. 388). Beijing: Chemical Industry Press.

Xiao Z. Journal of Southwest Agricultural University

Xie R. Journal of Systematics and Evolution

Yan H. Spectroscopy and Spectral Analysis

Yi S. Spectroscopy and Spectral Analysis

Zhang, Zhi-Min, Shan Chen, and Yi-Zeng Liang. "Baseline Correction Using Adaptive Iteratively Reweighted Penalized Least Squares." The Analyst 135.5 (2010): 1138. Crossref. Web.

Zhang Z. Spectroscopy and Spectral Analysis

Zhao H. Spectroscopy and Spectral Analysis

Zhou Z. Acta Horticulturae Sinica

Zhou X. Spectroscopy and Spectral Analysis

Zhou X. Spectroscopy and Spectral Analysis

Zhou Y. Spectroscopy and Spectral Analysis


ISSN PRINT: 1079-8587
ISSN ONLINE: 2326-005X
DOI PREFIX: 10.31209
10.1080/10798587 with T&F
IMPACT FACTOR: 0.652 (2017/2018)
Journal: 1995-Present


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